好氧氨氧化菌混培物的氨氧化动力学研究
Study on ammonia oxidation kinetics of ammonia oxidizer mixed culture
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摘要: 采用批式呼吸法求得好氧氨氧化菌产率系数为0.2119 mg COD/mg NH+4-NOD(或者0.7268 mg COD/mg NH+4-N)和氨氧化菌最大氨氮降解速率为0.1 mg NOD/(mg COD·h)(或者0.0292 mg N/(mg COD·h))。用间歇式批试验法,加入24 μmol/L NaN3抑制NO-2-N氧化,建立氨氧化反应动力学方程,得到氨氮半饱和系数为18.38 mg NOD/L(或者5.36 mg NH+4-N/L),DO半饱和系数为0.494 mg/L。对比参数值表明,用一步硝化动力学来描述氨氧化反应动力学模型是错误的。Abstract: The biomass yield coefficient and the maximum specific ammonium nitrogen consumption rate for autotrophic aerobic ammonia oxidation bacteria were 0.2119 mg COD produced/mg NH+4-NOD(or 0.7268 mg COD/mg NH+4-N) and 0.1 mg NOD/(mg COD·h)(or 0.0292 mg N/(mg COD·h)) from batch respirograms. The kinetics of aerobic ammonia oxidation was established by adding 24 μmol/L NaN3 in the sequence batch experiments. The kinetics parameters were determined, where the half saturate coefficient of NH+4-N was 18.38 mg NOD/L (or 5.36 mg NH+4-N/L) and the half saturate coefficient of DO was 0.494 mg/L. The results which were compared with kinetics parameters showed that single-step nitrification models erroneously describe NH+4-N to NO-2-N oxidation.
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Key words:
- aerobic ammonium oxidation bacteria /
- ammonium oxidization /
- kinetics
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