锶暴露对中华大蟾蜍蝌蚪生长发育的影响及其遗传毒性
Growth and Genotoxicity Effects Induced by Strontium Exposure in Tadpoles of B. gargarizans
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摘要: 为探究锶（Sr）对两栖动物的毒性效应，研究了不同浓度（0、0.2、2和20 mmol·L-1） SrCl2处理对中华大蟾蜍蝌蚪生长发育及遗传毒性的影响。结果表明，0.2 mmol·L-1 Sr2+对肝细胞谷胱甘肽S转移酶（GST）和过氧化氢酶（CAT）活性及金属硫蛋白（MT）含量没有显著影响。MT含量在2 mmol·L-1处理组最高，在20 mmol·L-1处理组低于对照组。这表明，Sr2+处理能诱导机体合成MT量的增加，但长时间高浓度处理会导致机体合成MT能力的损害。GST和CAT活性随着Sr2+暴露浓度上升而提高，表明环境中高浓度Sr2+可引起蝌蚪的氧化应激反应。低浓度的Sr2+处理对中华大蟾蜍蝌蚪不产生明显的遗传毒性，并且对生长发育有一定的促进作用；高浓度的Sr2+处理下，蝌蚪红细胞DNA的损伤程度显著增加，蝌蚪的生长发育受到显著抑制。Abstract: The toxic effect of strontium (Sr) on tadpole of B. gargarizans was investigated at three concentrations of SrCl2, i.e., 0.2, 2 and 20 mmol·L-1, respectively. Results show that low concentration of Sr2+ (0.2 mmol·L-1) does not produce significant effect on activities of glutathione S-transferase (GST) and catalase (CAT) enzymes and content of metallothionein (MT). The highest content of MT in hepatocytes was found in 2 mmol·L-1 Sr2+ treatment, while the content of MT in 20 mmol·L-1 Sr2+ treatment group was lower than that in the control. These results suggest that Sr2+ treatment could induce the synthesis of MT in vivo, but the synthesis of MT of tadpoles could be damaged by prolonged and high concentration of Sr2+ treatment. The increasing activities of GST and CAT enzymes with Sr2+ concentration suggest that high concentration of Sr2+ in the environment could induce oxidative stress in tadpoles. Low Sr2+ concentration did not cause obvious genotoxicity to tadpoles. However, the growth and development of tadpoles increase under low Sr2+ concentration. The DNA damage of tadpoles increased and the growth and development of tadpoles were significantly inhibited under high Sr2+ concentration.
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Key words:
- strontium /
- B.gargarizans /
- tadpoles /
- genotoxicity /
- physiological toxicity /
- development
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