近海沉积物DlPCBs对斑马鱼胚胎EROD和<i>cyp1a</i> mRNA的影响

生态毒理学报

近海沉积物DlPCBs对斑马鱼胚胎EROD和cyp1a mRNA的影响

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聂芳红, 蔡吉榛, 王小宁, 林红英, Ravi Gooneratne, Anthony Hay, 马驿, 巨向红, 郑锦庆, 陈进军. 近海沉积物DlPCBs对斑马鱼胚胎EROD和cyp1a mRNA的影响[J]. 生态毒理学报, 2016, 11(2): 364-368. doi: 10.7524/AJE.1673-5897.20150709001

引用本文:

聂芳红, 蔡吉榛, 王小宁, 林红英, Ravi Gooneratne, Anthony Hay, 马驿, 巨向红, 郑锦庆, 陈进军. 近海沉积物DlPCBs对斑马鱼胚胎EROD和cyp1a mRNA的影响[J]. 生态毒理学报, 2016, 11(2): 364-368. doi: 10.7524/AJE.1673-5897.20150709001

Nie Fanghong, Cai Jizhen, Wang Xiaoning, Lin Hongying, Gooneratne Ravi, Anthony Hay, Ma Yi, Ju Xianghong, Zheng Jinqing, Chen Jinjun. EROD Activity and cyp1a mRNA Expression in Zebrafish Embryo Exposed to Marine Sediment DlPCBs Extract[J]. Asian journal of ecotoxicology, 2016, 11(2): 364-368. doi: 10.7524/AJE.1673-5897.20150709001

Citation:

Nie Fanghong, Cai Jizhen, Wang Xiaoning, Lin Hongying, Gooneratne Ravi, Anthony Hay, Ma Yi, Ju Xianghong, Zheng Jinqing, Chen Jinjun. EROD Activity and cyp1a mRNA Expression in Zebrafish Embryo Exposed to Marine Sediment DlPCBs Extract[J]. Asian journal of ecotoxicology, 2016, 11(2): 364-368. doi: 10.7524/AJE.1673-5897.20150709001

近海沉积物DlPCBs对斑马鱼胚胎EROD和cyp1a mRNA的影响

1. 广东海洋大学食品科技学院, 湛江 524088;
2. 广东海洋大学农学院, 湛江 524088;
3. 林肯大学农业与生命学院, 基督城 7647, 新西兰;
4. 康奈尔大学农业与生命科学学院, 伊萨卡 14853, 美国

作者简介: 聂芳红(1969-),女,硕士,高级实验师,研究方向为食品毒理学,E-mail:15913577717@163.com

通讯作者: 陈进军, E-mail: jjchen777@aliyun.com

基金项目:
广东省科技计划国际合作项目(2010B050600004,2016A050502062)
中图分类号: X171.5

EROD Activity and cyp1a mRNA Expression in Zebrafish Embryo Exposed to Marine Sediment DlPCBs Extract

1. Food Sci-Tech College, Guangdong Ocean University, Zhanjiang 524088, China;
2. Agricultural College, Guangdong Ocean University, Zhanjiang 524088, China;
3. Faculty of Agriculture & Life Sciences, Lincoln University, Christchurch 7647, New Zealand;
4. College of Agriculture and Life Sciences, Cornell University, Ithaca 14853, USA

Corresponding author: Chen Jinjun, jjchen777@aliyun.com

Fund Project:

摘要: 为探究湛江近海域海洋沉积物中类二噁英多氯联苯(dioxin-like polychlorinated biphenyls,DlPCBs)的生物学毒性效应，选取湛江近海域2个地点(近工业区TS和近生活区JSW)采集沉积物样品，制备近海域沉积物DlPCBs提取物，将斑马鱼胚胎暴露于不同浓度的DlPCBs提取物，测定斑马鱼胚胎7-乙氧基异吩恶唑脱乙基酶(ethoxyresorufin-O-deethylase,EROD)活性和cyp1a mRNA相对表达量。结果发现，JSW采样点DlPCBs提取物染毒组EROD酶活性变化与TS采样点DlPCBs提取物一致，在各染毒浓度下，斑马鱼仔鱼EROD酶活性为对照组的1.1～1.8倍。TS和JSW采样点不同浓度DlPCBs提取物暴露斑马鱼胚胎96 h后，使斑马鱼仔鱼cyp1a mRNA相对表达量是对照组的3.36～19.45倍。说明一定浓度的近海沉积物DlPCBs能诱导斑马鱼仔鱼EROD酶活性和cyp1a mRNA表达量升高，且呈现浓度－效应关系。
- 类二噁英多氯联苯 /
- 斑马鱼胚胎 /
- EROD酶 /
- cyp1a mRNA /
- 近海沉积物
Abstract: To study the bio-toxicity of dioxin-like polychlorinated biphenyls (DlPCBs) in marine sediment, the sediments from Zhanjiang industrial and living area (TS and JSW) were collected and extracted focusing on DlPCBs, respectively. Then a range of zebrafish embryos were exposed to different concentrations of the sediment DlPCBs extracts to examine the ethoxyresorufin-O-deethylase (EROD) activity and the expression of cyp1a mRNA. The results showed that the DlPCBs extract from TS and JSW sediments had similarity to cause marked induction of EROD in the zebrafish embryos.The EROD activities in zebrafish embryos induced by different concentrations of the DlPCBs extract were 1.1 to 1.8 times higher than that in the control embryos, respectively, and after the zebrafish embryos were exposed to different concentrations of the DlPCBs for 96 h, the juvenile cyp1a mRNA relative expressions were significantly enhanced by 3.36 to 19.45 times of that in the control group, respectively. It is indicated that certain concentrations of the DlPCBs extract increased EROD activity and cyp1a mRNA in zebrafish larvae, with a concentration-effect relationship.
- dioxin-like polychlorinated biphenyls /
- zebrafish embryo /
- EROD /
- cyp1a mRNA /
- marine sediment

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聂芳红, 蔡吉榛, 王小宁, 林红英, Ravi Gooneratne, Anthony Hay, 马驿, 巨向红, 郑锦庆, 陈进军. 近海沉积物DlPCBs对斑马鱼胚胎EROD和cyp1a mRNA的影响[J]. 生态毒理学报, 2016, 11(2): 364-368. doi: 10.7524/AJE.1673-5897.20150709001

引用本文:

聂芳红, 蔡吉榛, 王小宁, 林红英, Ravi Gooneratne, Anthony Hay, 马驿, 巨向红, 郑锦庆, 陈进军. 近海沉积物DlPCBs对斑马鱼胚胎EROD和cyp1a mRNA的影响[J]. 生态毒理学报, 2016, 11(2): 364-368. doi: 10.7524/AJE.1673-5897.20150709001

Nie Fanghong, Cai Jizhen, Wang Xiaoning, Lin Hongying, Gooneratne Ravi, Anthony Hay, Ma Yi, Ju Xianghong, Zheng Jinqing, Chen Jinjun. EROD Activity and cyp1a mRNA Expression in Zebrafish Embryo Exposed to Marine Sediment DlPCBs Extract[J]. Asian journal of ecotoxicology, 2016, 11(2): 364-368. doi: 10.7524/AJE.1673-5897.20150709001

Citation:

Nie Fanghong, Cai Jizhen, Wang Xiaoning, Lin Hongying, Gooneratne Ravi, Anthony Hay, Ma Yi, Ju Xianghong, Zheng Jinqing, Chen Jinjun. EROD Activity and cyp1a mRNA Expression in Zebrafish Embryo Exposed to Marine Sediment DlPCBs Extract[J]. Asian journal of ecotoxicology, 2016, 11(2): 364-368. doi: 10.7524/AJE.1673-5897.20150709001

近海沉积物DlPCBs对斑马鱼胚胎EROD和cyp1a mRNA的影响

通讯作者: 陈进军, E-mail: jjchen777@aliyun.com

作者简介: 聂芳红(1969-),女,硕士,高级实验师,研究方向为食品毒理学,E-mail:15913577717@163.com
1. 广东海洋大学食品科技学院, 湛江 524088;

2. 广东海洋大学农学院, 湛江 524088;

3. 林肯大学农业与生命学院, 基督城 7647, 新西兰;

4. 康奈尔大学农业与生命科学学院, 伊萨卡 14853, 美国

收稿日期: 2015-07-09

基金项目:

广东省科技计划国际合作项目(2010B050600004,2016A050502062)

关键词:

摘要: 为探究湛江近海域海洋沉积物中类二噁英多氯联苯(dioxin-like polychlorinated biphenyls,DlPCBs)的生物学毒性效应，选取湛江近海域2个地点(近工业区TS和近生活区JSW)采集沉积物样品，制备近海域沉积物DlPCBs提取物，将斑马鱼胚胎暴露于不同浓度的DlPCBs提取物，测定斑马鱼胚胎7-乙氧基异吩恶唑脱乙基酶(ethoxyresorufin-O-deethylase,EROD)活性和cyp1a mRNA相对表达量。结果发现，JSW采样点DlPCBs提取物染毒组EROD酶活性变化与TS采样点DlPCBs提取物一致，在各染毒浓度下，斑马鱼仔鱼EROD酶活性为对照组的1.1～1.8倍。TS和JSW采样点不同浓度DlPCBs提取物暴露斑马鱼胚胎96 h后，使斑马鱼仔鱼cyp1a mRNA相对表达量是对照组的3.36～19.45倍。说明一定浓度的近海沉积物DlPCBs能诱导斑马鱼仔鱼EROD酶活性和cyp1a mRNA表达量升高，且呈现浓度－效应关系。

English Abstract

EROD Activity and cyp1a mRNA Expression in Zebrafish Embryo Exposed to Marine Sediment DlPCBs Extract

Corresponding author: Chen Jinjun, jjchen777@aliyun.com

1. Food Sci-Tech College, Guangdong Ocean University, Zhanjiang 524088, China;
2. Agricultural College, Guangdong Ocean University, Zhanjiang 524088, China;
3. Faculty of Agriculture & Life Sciences, Lincoln University, Christchurch 7647, New Zealand;
4. College of Agriculture and Life Sciences, Cornell University, Ithaca 14853, USA

Received Date: 2015-07-09

Fund Project:

Keywords:

Abstract: To study the bio-toxicity of dioxin-like polychlorinated biphenyls (DlPCBs) in marine sediment, the sediments from Zhanjiang industrial and living area (TS and JSW) were collected and extracted focusing on DlPCBs, respectively. Then a range of zebrafish embryos were exposed to different concentrations of the sediment DlPCBs extracts to examine the ethoxyresorufin-O-deethylase (EROD) activity and the expression of cyp1a mRNA. The results showed that the DlPCBs extract from TS and JSW sediments had similarity to cause marked induction of EROD in the zebrafish embryos.The EROD activities in zebrafish embryos induced by different concentrations of the DlPCBs extract were 1.1 to 1.8 times higher than that in the control embryos, respectively, and after the zebrafish embryos were exposed to different concentrations of the DlPCBs for 96 h, the juvenile cyp1a mRNA relative expressions were significantly enhanced by 3.36 to 19.45 times of that in the control group, respectively. It is indicated that certain concentrations of the DlPCBs extract increased EROD activity and cyp1a mRNA in zebrafish larvae, with a concentration-effect relationship.

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