利用SOS/umu测试方法鉴定沙颍河河水中的遗传毒性物质

张建超; 马福俊; 胡建英

doi:10.7524/AJE.1673-5897.20121224001

生态毒理学报

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张建超, 马福俊, 胡建英. 利用SOS/umu测试方法鉴定沙颍河河水中的遗传毒性物质[J]. 生态毒理学报, 2013, 8(3): 344-349. doi: 10.7524/AJE.1673-5897.20121224001

引用本文:

张建超, 马福俊, 胡建英. 利用SOS/umu测试方法鉴定沙颍河河水中的遗传毒性物质[J]. 生态毒理学报, 2013, 8(3): 344-349. doi: 10.7524/AJE.1673-5897.20121224001

Zhang Jianchao, Ma Fujun, Hu Jianying. SOS/umu Test for Detection of Genotoxins in Water of Shaying River[J]. Asian Journal of Ecotoxicology, 2013, 8(3): 344-349. doi: 10.7524/AJE.1673-5897.20121224001

Citation:

Zhang Jianchao, Ma Fujun, Hu Jianying. SOS/umu Test for Detection of Genotoxins in Water of Shaying River[J]. Asian Journal of Ecotoxicology, 2013, 8(3): 344-349. doi: 10.7524/AJE.1673-5897.20121224001

利用SOS/umu测试方法鉴定沙颍河河水中的遗传毒性物质

1. 山东师范大学生命科学学院, 济南 250014;
2. 北京大学城市与环境学院, 北京 100871

作者简介: 张建超(1988-),女,硕士,研究方向为食品生物技术,E-mail:ahcomeon@126.com

基金项目:
科技部863项目(2012AA062802)
中图分类号: X171.5

SOS/umu Test for Detection of Genotoxins in Water of Shaying River

1. College of Life Science, Shandong Normal University, Ji'nan 250014, China;
2. College of Urban and Environmental Sciences, Peking University, Beijing 100871, China
Fund Project:

摘要: 采用HPLC分割导向的SOS/umu测试方法鉴定了沙颍河河水中的遗传毒性物质。当采用TA1535/pSK1002菌株测定HPLC分割的各馏分时,如果不经大鼠肝微粒体酶(S9)代谢活化,只有馏分F10显示遗传毒性,加入大鼠肝微粒体酶代谢活化后,馏分F10和馏分F15都显示有遗传毒性,说明河水中存在某些需经过大鼠肝微粒体酶代谢活化才能显示出遗传毒性的物质。当采用过量表达O-乙酰转移酶(O-AT),对芳香胺类物质和硝基芳烃化合物有特殊响应的NM2009菌株测定时,馏分F8、F9和F10均呈现遗传毒性;特别是对于馏分F10,用NM2009菌株测定的遗传毒性远高于原始菌,说明这3个馏分中都含有芳香胺类或硝基芳烃类物质。
- 地表水 /
- SOS/umu实验 /
- 遗传毒性 /
- 芳香胺 /
- 硝基芳烃
Abstract: In the present study, HPLC fractionation directed SOS/umu assay was performed to identify the genotoxic chemicals in Shaying River water samples. After HPLC fractionation, every fraction was tested. Genotoxic activity was observed only in Fraction 10 upon using TA1535/pSK1002 strain without rat liver microsomes (S9) addition, while genotoxic activities were observed in both Fraction 10 and 15 by using same strain with rat liver microsomes addition, indicating that some genotoxins in water samples elicited their genotoxicity under rat liver microsomes metabolic activation. NM2009 strain can over-express O-AT and show specific response to aromatic amines and nitro-aromatic compounds. When NM2009 strain was used to test, the genotoxic activities were detected in Fractions 8, 9 and 10. Especially, the genotoxic activity of Fraction 10 was greater than that when using TA1535/pSK1002 strain to test. These results indicated that the major causative agents of Fraction 8, 9, and 10 should be aromatic amines or nitroarenes.
- surface water /
- SOS/umu test /
- genotoxicity /
- aromatic amines /
- nitro-aromatic compounds

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张建超, 马福俊, 胡建英. 利用SOS/umu测试方法鉴定沙颍河河水中的遗传毒性物质[J]. 生态毒理学报, 2013, 8(3): 344-349. doi: 10.7524/AJE.1673-5897.20121224001

引用本文:

张建超, 马福俊, 胡建英. 利用SOS/umu测试方法鉴定沙颍河河水中的遗传毒性物质[J]. 生态毒理学报, 2013, 8(3): 344-349. doi: 10.7524/AJE.1673-5897.20121224001

Citation:

利用SOS/umu测试方法鉴定沙颍河河水中的遗传毒性物质

作者简介: 张建超(1988-),女,硕士,研究方向为食品生物技术,E-mail:ahcomeon@126.com
1. 山东师范大学生命科学学院, 济南 250014;

2. 北京大学城市与环境学院, 北京 100871

收稿日期: 2012-12-24

基金项目:

科技部863项目(2012AA062802)

关键词:

摘要: 采用HPLC分割导向的SOS/umu测试方法鉴定了沙颍河河水中的遗传毒性物质。当采用TA1535/pSK1002菌株测定HPLC分割的各馏分时,如果不经大鼠肝微粒体酶(S9)代谢活化,只有馏分F10显示遗传毒性,加入大鼠肝微粒体酶代谢活化后,馏分F10和馏分F15都显示有遗传毒性,说明河水中存在某些需经过大鼠肝微粒体酶代谢活化才能显示出遗传毒性的物质。当采用过量表达O-乙酰转移酶(O-AT),对芳香胺类物质和硝基芳烃化合物有特殊响应的NM2009菌株测定时,馏分F8、F9和F10均呈现遗传毒性;特别是对于馏分F10,用NM2009菌株测定的遗传毒性远高于原始菌,说明这3个馏分中都含有芳香胺类或硝基芳烃类物质。

English Abstract

SOS/umu Test for Detection of Genotoxins in Water of Shaying River

1. College of Life Science, Shandong Normal University, Ji'nan 250014, China;
2. College of Urban and Environmental Sciences, Peking University, Beijing 100871, China

Received Date: 2012-12-24

Fund Project:

Keywords:

Abstract: In the present study, HPLC fractionation directed SOS/umu assay was performed to identify the genotoxic chemicals in Shaying River water samples. After HPLC fractionation, every fraction was tested. Genotoxic activity was observed only in Fraction 10 upon using TA1535/pSK1002 strain without rat liver microsomes (S9) addition, while genotoxic activities were observed in both Fraction 10 and 15 by using same strain with rat liver microsomes addition, indicating that some genotoxins in water samples elicited their genotoxicity under rat liver microsomes metabolic activation. NM2009 strain can over-express O-AT and show specific response to aromatic amines and nitro-aromatic compounds. When NM2009 strain was used to test, the genotoxic activities were detected in Fractions 8, 9 and 10. Especially, the genotoxic activity of Fraction 10 was greater than that when using TA1535/pSK1002 strain to test. These results indicated that the major causative agents of Fraction 8, 9, and 10 should be aromatic amines or nitroarenes.