双酚A对斑马鱼成鱼肝脏功能基因转录调控的影响
Effects of Bisphenol A on the Transcriptions of Typical Functional Genes in Adult Zebrafish (Danio rerio) Liver
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摘要: 运用实时定量即时聚合链式反应RT-PCR方法,研究了双酚A对斑马鱼成鱼肝脏的功能基因转录水平的影响。结果显示,28 d半静水暴露以后,FGF8A和STAU 2表现出传统的正剂量-效应关系,CLDN 1和CALCA的表达为倒剂量-抑制效应关系,SP 4基因在15 μg·L-1双酚A时表现为显著抑制效应,但是在150和1 500 μg·L-1却表现为显著的诱导效应。试验进一步探讨了这些表达异常的基因在双酚A干扰去除后能否自我调节和修复。结果显示,CLDN 1能恢复到正常表达水平,而其他4个基因在低剂量组却维持原来的高毒性作用水平甚至表现出更高的毒性作用。研究证明双酚A对斑马鱼成鱼肝脏的基因转录调控具有一定的非单调剂量-效应作用。Abstract: The effects of bisphenol A (BPA) on the transcriptions of typical function genes in adult zebrafish liver were investigated by means of real-time quantitative polymerase chain reaction(RT-PCR). The results showed that monotonic dose-response curves were existed in the transcriptions of FGF8A and STAU 2 after 28 days of BPA exposure, however, non-monotonic curves were found for CLDN 1 and CALCA genes. SP 4 was significantly inhibited in the group of 15 g·L-1 BPA but stimulated in the group of 150 and 1 500 g·L-1 BPA. The self regulation and repair function of the abnormal expressed genes were further discussed when the BPA interference was removed. The results showed that the expression of CLDN 1 could be recovered to normal level. While the other four genes couldn't recover, at low dose groups, they maintained the original high toxicity levels and even perform higher toxicities. The research testified that some non monotonic dose-effect existed in BPA and the gene transcriptions in adult zebrafish liver.
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Key words:
- bisphenol a /
- zebrafish liver /
- function gene /
- non monotonic dose -effect /
- repair
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