摘要:
多环芳烃(PAHs)是一类广泛存在于环境中的持久性有机污染物,大多具有潜在毒性、致癌性及致畸诱变作用。选取马氏珠母贝(Pinctada martensi)作为模式动物,研究芘对马氏珠母贝D型面盘幼虫发育的影响。将马氏珠母贝D型面盘幼虫暴露于不同浓度(2、4、8、16和32 μg·L-1)芘(pyrene)中,在暴露后第12、24、36、48、60、72和84小时,分别测定其死亡率和畸形率,并观察形态学的变化。结果表明,芘对D型面盘幼虫的发育有显著影响,同一浓度组的死亡率与时间呈正相关。在2~32 μg·L-1浓度范围内,不同暴露时间段,死亡率与浓度呈正相关。芘暴露对马氏珠母贝D型面盘幼虫的形态也有显著影响,随着芘浓度的增大幼虫畸形的比例增高,并呈现明显的剂量-效应关系,其中8和32 μg·L-1浓度组的响应最敏感。同时,芘暴露对马氏珠母贝D型面盘幼虫的半数致死浓度(LC50)随着时间的推移逐渐降低,60 h后趋于稳定,LC50值为43.50 μg·L-1。研究结果表明,芘暴露能影响马氏珠母贝D型面盘幼虫的发育,并可能对其种群结构产生不利影响。
Abstract:
Polycyclic aromatic hydrocarbons (PAHs) is one of persistent organic pollutants (POPs) with carcinogenicity, teratogenicity and mutagenicity, widespread throughout the environment. In this paper, the impacts of pyrene (Py) on the development and morphology of D-shaped veliger larvae of Pinctada martensi were studied. Mortality and aberration rate and the changes of morphology of D-shaped veliger larvae were recorded after Pinctada martensi exposed to different concentrations (2, 4, 8, 16 and 32 μg·L-1) of Py for 12, 24, 36, 48, 72, and 84 hours. The results indicated that Py has significant toxic effect on the development of D-shaped veliger larvae in a time-dependent manner. The observed mortality was positively correlated to the concentrations of Py in the range of 2~32 μg·L-1at different exposure times. Moreover, with increasing concentrations of Py, the percentages of abnormal individual increased significantly in a dose-dependent manner. Exposed to concentrations of 8 μg·L-1 and 32 μg·L-1, larvae were much more sensitive than that exposed to other concentration levels. With the passage of time, LC 50 firstly decreased then tended to be constant. The recorded 60 h-LC50 was 43.50 μg·L-1. It can be concluded that Py exposure affected the development of D-shaped veliger larvae and could cause some adverse effect on the population structure of Pinctada martensi.
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