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石墨烯量子点荧光探针的合成及其对TNT痕量检测的应用

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席小倩, 漆天瑶, 周杨群, 张慧慧, 陈倩云, 刘辰辰, 黄宸, 高大明, 陈红. 石墨烯量子点荧光探针的合成及其对TNT痕量检测的应用[J]. 环境工程学报, 2018, 12(5): 1310-1317. doi: 10.12030/j.cjee.201709104
引用本文: 席小倩, 漆天瑶, 周杨群, 张慧慧, 陈倩云, 刘辰辰, 黄宸, 高大明, 陈红. 石墨烯量子点荧光探针的合成及其对TNT痕量检测的应用[J]. 环境工程学报, 2018, 12(5): 1310-1317. doi: 10.12030/j.cjee.201709104
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XI Xiaoqian, QI Tianyao, ZHOU Yangqun, ZHANG Huihui, CHEN Qianyun, LIU Chenchen, HUANG Chen, GAO Daming, CHEN Hong. Synthesis of graphene quantum dots (GQDs) fluorescence probe and its application of trace detection towards TNT[J]. Chinese Journal of Environmental Engineering, 2018, 12(5): 1310-1317. doi: 10.12030/j.cjee.201709104
Citation: XI Xiaoqian, QI Tianyao, ZHOU Yangqun, ZHANG Huihui, CHEN Qianyun, LIU Chenchen, HUANG Chen, GAO Daming, CHEN Hong. Synthesis of graphene quantum dots (GQDs) fluorescence probe and its application of trace detection towards TNT[J]. Chinese Journal of Environmental Engineering, 2018, 12(5): 1310-1317. doi: 10.12030/j.cjee.201709104
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石墨烯量子点荧光探针的合成及其对TNT痕量检测的应用

  • 1.

    合肥学院生物与环境工程系，合肥 230601

  • 2.

    合肥学院化学与材料工程系，合肥 230601

  • 基金项目:

    国家自然科学基金资助项目(21606066，21075026)

    安徽省自然科学基金资助项目(1708085QB46)

    安徽省大学生创新创业项目(201611059098)

Synthesis of graphene quantum dots (GQDs) fluorescence probe and its application of trace detection towards TNT

  • 1.

    Department of Biology and Environmental Engineering, Hefei University, Hefei 230601, China

  • 2.

    Department of Chemistry and Materials Engineering, Hefei University, Hefei 230601, China

  • Fund Project:

  • 摘要: 采用石墨制备出氧化石墨稀（GO），利用水合肼还原GO，将还原的GO与二甲基甲酰胺（DMF）反应，制备出绿色发射波长的石墨烯量子点（graphene quantum dots，GQDs）荧光探针，分别用拉曼、UV-vis、FT-IR、SEM和Zeta电位仪等对其性质进行表征和研究。由于GQDs荧光探针表面富电子，而目标分析物TNT分子的3个缺电子硝基是吸电子基团，二者空间相互接近时，发生荧光共振能量转移，导致GQDs荧光探针的荧光强度发生改变，实现对痕量目标分子TNT的检测。结果表明，所制备GQDs荧光探针实现对TNT分子的高选择性识别，高敏感性检测，检出限为1.0×10-9 mol·L-1，猝灭常数为7 965 L·mol-1。基于荧光共振能量转移原理合成GQDs荧光探针实现对痕量TNT爆炸物的选择性识别和敏感性检测。
    Abstract: Firstly, graphene oxide was obtained by graphite through the improved Hummers method. Graphene was prepared through reduction reaction of the aforementioned GO with hydrazine hydrate. Subsequently, GQDs fluorescence probe with emission green wavelengths was achieved by the resultant graphene dissolved the N, N-dimethylformamide (DMF) solution using the hydrothermal method. Moreover, the physical properties for GQDs fluorescence probe were characterized with Raman spectroscopy, UV-vis, FT-IR, SEM and Zeta potential analysis, respectively. Meanwhile, GQDs fluorescence probe with the rich-electron surface approached to TNT molecule with deficient-electron three nitro groups at spatial position to suppress the fluorescence intensity of probe based on fluorescence resonance energy transfer (FRET). The results demonstrated that the synthesized GQDs fluorescence probe synthesized has the high selective recognition and sensible detection to TNT molecule, and the trace detection limit and quenching constant to TNT is 1.0×10-9 mol·L-1 and 7 965 L·mol-1, respectively. The synthesized GQDs fluorescence probe was carried out to selectively recognize and sensibly detect the trace TNT explosive based on FRET.
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  • 刊出日期:  2018-05-19

石墨烯量子点荧光探针的合成及其对TNT痕量检测的应用

  • 1. 合肥学院生物与环境工程系，合肥 230601
  • 2. 合肥学院化学与材料工程系，合肥 230601
基金项目:

国家自然科学基金资助项目(21606066，21075026)

安徽省自然科学基金资助项目(1708085QB46)

安徽省大学生创新创业项目(201611059098)

摘要: 采用石墨制备出氧化石墨稀（GO），利用水合肼还原GO，将还原的GO与二甲基甲酰胺（DMF）反应，制备出绿色发射波长的石墨烯量子点（graphene quantum dots，GQDs）荧光探针，分别用拉曼、UV-vis、FT-IR、SEM和Zeta电位仪等对其性质进行表征和研究。由于GQDs荧光探针表面富电子，而目标分析物TNT分子的3个缺电子硝基是吸电子基团，二者空间相互接近时，发生荧光共振能量转移，导致GQDs荧光探针的荧光强度发生改变，实现对痕量目标分子TNT的检测。结果表明，所制备GQDs荧光探针实现对TNT分子的高选择性识别，高敏感性检测，检出限为1.0×10-9 mol·L-1，猝灭常数为7 965 L·mol-1。基于荧光共振能量转移原理合成GQDs荧光探针实现对痕量TNT爆炸物的选择性识别和敏感性检测。

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