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一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响

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吴晓娜, 王助贫, 谢恩, 任梦丽, 刘婷婷, 郑蕾, 丁爱中. 一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响[J]. 环境工程学报, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224
引用本文: 吴晓娜, 王助贫, 谢恩, 任梦丽, 刘婷婷, 郑蕾, 丁爱中. 一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响[J]. 环境工程学报, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224
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WU Xiaona, WANG Zhupin, XIE En, REN Mengli, LIU Tingting, ZHENG Lei, DING Aizhong. Screening of one strain of denitrifying phosphorus accumulation bacteria (DPAB) and inhibitions effects of nitrogen-phosphorus removal[J]. Chinese Journal of Environmental Engineering, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224
Citation: WU Xiaona, WANG Zhupin, XIE En, REN Mengli, LIU Tingting, ZHENG Lei, DING Aizhong. Screening of one strain of denitrifying phosphorus accumulation bacteria (DPAB) and inhibitions effects of nitrogen-phosphorus removal[J]. Chinese Journal of Environmental Engineering, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224
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一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响

  • 1.

    北京师范大学水科学研究院,北京100875

  • 2.

    北控水务集团有限公司,北京 100102

  • 3.

    中国农业大学水利与土木工程学院,北京 100083

  • 基金项目:

    北京市科技计划项目(D161100000216001)

    国家自然科学基金资助项目(51509003)

Screening of one strain of denitrifying phosphorus accumulation bacteria (DPAB) and inhibitions effects of nitrogen-phosphorus removal

  • 1.

    College of Water Sciences,Beijing Normal University, Beijing 100875, China

  • 2.

    Beijing Enterprises Water Group Limited, Beijing 100102, China

  • 3.

    College of Water Resources & Civil Engineering, China Agricultural University, Beijing 100083, China

  • Fund Project:

  • 摘要: 以环形推流一体化氧化沟反应器厌氧段污泥为接种污泥,通过吸磷实验、硝酸盐还原产气实验、异染颗粒染色实验及PHB染色实验从接种污泥中筛选得到一株能实现同步脱氮除磷菌株N-12；经过16S rDNA测序鉴定,确定该菌株为不动杆菌属(Acinetobacter)。绘制该菌株的生长曲线,并设置0%、1%、2%、5%、10%和15%等6种不同初始接种比,以接种比作为微生物生物量的衡量参数,研究不同接种比对反硝化聚磷菌脱氮除磷效应的影响,确定菌株最佳接种比。实验结果表明:初始接种比2%体系的脱氮除磷效果最好；利用SPSS statistics 19统计软件对磷酸盐去除率、硝态氮去除率、OD600值及pH进行相关性分析,表明磷酸盐去除率和硝酸盐去除率显著正相关(Person相关系数=0.826,P600值及pH值都显著正相关。
    Abstract: In order to attribute to effective and low energy-consumption sewage treatment technology, we screened one strain of DPAB and determined the best inoculation ratio experiments. Using anaerobic sludge as the inoculated sludge, one strain, N-12, was obtained through a series of experiments, including phosphate uptake, nitrate reduction to gas, auxiliary dye granular straining and PHB. Besides, the strain was identified as Acinetobacter by 16S rDNA gene analysis. Then, draw the growth curve and set six different initial inoculation ratios as the measurement of microbial biomass, 0%, 1%, 2%, 5%, 10% and 15%, respectively, aim to study the inhibitions effects of nitrogen-phosphorus removal. The results showed that the most efficient was the 2% system. Results of the correlation analysis, among the removal rates of phosphate and nitrate, OD600 and pH, showed that there was a significant positive correlation coefficient between phosphate removal rate and nitrate removal rate (Person correlation coefficient=0.826, P600 and pH.
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  • 刊出日期:  2018-02-08

一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响

  • 1. 北京师范大学水科学研究院,北京100875
  • 2. 北控水务集团有限公司,北京 100102
  • 3. 中国农业大学水利与土木工程学院,北京 100083
基金项目:

北京市科技计划项目(D161100000216001)

国家自然科学基金资助项目(51509003)

摘要: 以环形推流一体化氧化沟反应器厌氧段污泥为接种污泥,通过吸磷实验、硝酸盐还原产气实验、异染颗粒染色实验及PHB染色实验从接种污泥中筛选得到一株能实现同步脱氮除磷菌株N-12；经过16S rDNA测序鉴定,确定该菌株为不动杆菌属(Acinetobacter)。绘制该菌株的生长曲线,并设置0%、1%、2%、5%、10%和15%等6种不同初始接种比,以接种比作为微生物生物量的衡量参数,研究不同接种比对反硝化聚磷菌脱氮除磷效应的影响,确定菌株最佳接种比。实验结果表明:初始接种比2%体系的脱氮除磷效果最好；利用SPSS statistics 19统计软件对磷酸盐去除率、硝态氮去除率、OD600值及pH进行相关性分析,表明磷酸盐去除率和硝酸盐去除率显著正相关(Person相关系数=0.826,P600值及pH值都显著正相关。

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