高级搜索

一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响

,  ,  ,  ,  ,  , 

downloadPDF

上一篇

下一篇

吴晓娜, 王助贫, 谢恩, 任梦丽, 刘婷婷, 郑蕾, 丁爱中. 一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响[J]. 环境工程学报, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224
引用本文: 吴晓娜, 王助贫, 谢恩, 任梦丽, 刘婷婷, 郑蕾, 丁爱中. 一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响[J]. 环境工程学报, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224
shu
WU Xiaona, WANG Zhupin, XIE En, REN Mengli, LIU Tingting, ZHENG Lei, DING Aizhong. Screening of one strain of denitrifying phosphorus accumulation bacteria (DPAB) and inhibitions effects of nitrogen-phosphorus removal[J]. Chinese Journal of Environmental Engineering, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224
Citation: WU Xiaona, WANG Zhupin, XIE En, REN Mengli, LIU Tingting, ZHENG Lei, DING Aizhong. Screening of one strain of denitrifying phosphorus accumulation bacteria (DPAB) and inhibitions effects of nitrogen-phosphorus removal[J]. Chinese Journal of Environmental Engineering, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224
shu

一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响

  • 1.

    北京师范大学水科学研究院,北京100875

  • 2.

    北控水务集团有限公司,北京 100102

  • 3.

    中国农业大学水利与土木工程学院,北京 100083

  • 基金项目:

    北京市科技计划项目(D161100000216001)

    国家自然科学基金资助项目(51509003)

Screening of one strain of denitrifying phosphorus accumulation bacteria (DPAB) and inhibitions effects of nitrogen-phosphorus removal

  • 1.

    College of Water Sciences,Beijing Normal University, Beijing 100875, China

  • 2.

    Beijing Enterprises Water Group Limited, Beijing 100102, China

  • 3.

    College of Water Resources & Civil Engineering, China Agricultural University, Beijing 100083, China

  • Fund Project:

  • 摘要: 以环形推流一体化氧化沟反应器厌氧段污泥为接种污泥,通过吸磷实验、硝酸盐还原产气实验、异染颗粒染色实验及PHB染色实验从接种污泥中筛选得到一株能实现同步脱氮除磷菌株N-12；经过16S rDNA测序鉴定,确定该菌株为不动杆菌属(Acinetobacter)。绘制该菌株的生长曲线,并设置0%、1%、2%、5%、10%和15%等6种不同初始接种比,以接种比作为微生物生物量的衡量参数,研究不同接种比对反硝化聚磷菌脱氮除磷效应的影响,确定菌株最佳接种比。实验结果表明:初始接种比2%体系的脱氮除磷效果最好；利用SPSS statistics 19统计软件对磷酸盐去除率、硝态氮去除率、OD600值及pH进行相关性分析,表明磷酸盐去除率和硝酸盐去除率显著正相关(Person相关系数=0.826,P600值及pH值都显著正相关。
    Abstract: In order to attribute to effective and low energy-consumption sewage treatment technology, we screened one strain of DPAB and determined the best inoculation ratio experiments. Using anaerobic sludge as the inoculated sludge, one strain, N-12, was obtained through a series of experiments, including phosphate uptake, nitrate reduction to gas, auxiliary dye granular straining and PHB. Besides, the strain was identified as Acinetobacter by 16S rDNA gene analysis. Then, draw the growth curve and set six different initial inoculation ratios as the measurement of microbial biomass, 0%, 1%, 2%, 5%, 10% and 15%, respectively, aim to study the inhibitions effects of nitrogen-phosphorus removal. The results showed that the most efficient was the 2% system. Results of the correlation analysis, among the removal rates of phosphate and nitrate, OD600 and pH, showed that there was a significant positive correlation coefficient between phosphate removal rate and nitrate removal rate (Person correlation coefficient=0.826, P600 and pH.
  • 加载中
  • [1] GILLOR O, HADAS O, POST A F, et al.Phosphorus and nitrogen in a monomictic freshwater lake: Employing cyanobacterial bioreporters to gain new insights into nutrient bioavailability[J].Freshwater Biology,2010,5(6):1182-1190
    [2] SHIELDS F D, TESTA I S, COOPER C M.Nitrogen and phosphorus levels in the yazoo river basin, mississippi[J].Ecohydrology, 2009,2(3):270-278
    [3] XIA S B, LIU J X.An innovative integrated oxidation ditch with vertical circle(IODVC) for wastewater treatment[J].Journal of Environmental Science,2004,6(3):367-370
    [4] KUBA T, SMOLDERS G, LOOSDRECHT M C M V.Biological phosphorus removal from wastewater by anaerobic-anoxic sequencing batch reactor[J].Waterence & Technology,1993,7(5/6):241-252
    [5] VARGAS M, GUISASOLA A, ARTIGUES A, et al.Comparison of a nitrite-based anaerobic-anoxic EBPR system with propionate or acetate as electron donors[J].Process Biochemistry,2011,6(3):714-720
    [6] TSUNEDA S, OHNO T, SOEJIMA K, et al.Simultaneous nitrogen and phosphorus removal using denitrifying phosphate-accumulating organisms in a sequencing batch reactor[J].Biochemical Engineering Journal,2006,7(3):191-196
    [7] 林雨倩,白少元,王敦球,等.城市污水处理厂反硝化聚磷菌的聚磷持久性[J].环境工程学报,2012,6(11):4021-4026
    [8] 李慧,刘丹丹,陈文清.反硝化聚磷菌的筛选及脱氮除磷特性[J].环境工程,2016,4(4):25-28
    [9] BORTONE G, LIBELLI S M, TILCHE A, et al.Anoxic phosphate uptake in the dephanox process[J].Water Science & Technology,1999,0(4/5):177-185
    [10] KUBA T, LOOSRRECHT M C M V, HEIJNEN J J.Phosphorus and nitrogen removal with minimal COD requirement by integration of denitrifying dephosphatation and nitrification in a two-sludge system[J].Water Research,1996,0(7):1702-1710
    [11] 杨志愿,邱业先,李孝坤,等.三株反硝化聚磷菌的筛选及其生理生化特性的鉴定[J].环境科技,2009,2(3):36-40
    [12] 马放,王春丽,王立立.高效反硝化聚磷菌株的筛选及其生物学特性[J].哈尔滨工程大学学报,2007,8(6):631-635
    [13] 刘丽,张永军,许长征,等.一种改良的CTAB法提取产多糖真菌DNA[J].中国生物工程杂志,2014,4(5):75-79
    [14] 易润华,朱西儒,周而勋.简化CTAB法快速微量提取丝状真菌DNA[J].广东海洋大学学报,2003,3(6):72-73
    [15] 黄绍辉,方炎明.改进的SDS-CTAB法提取濒危植物连香树总DNA[J].植物科学学报,2007,5(1):98-101
    [16] 王凡,洪葵.CTAB法提取野野村菌基因组DNA[J].微生物学通报,2010,7(8):1211-1215
    [17] ROGGENBUCK M, SCHNELL I B, BLOM N, et al.The microbiome of new world vultures[J].Nature Communications,2014,5:5498
    [18] DE M S, ZOTHANSANGA, PANDA A K, et al.MiSeq HV4 16S rRNA gene analysis of bacterial community composition among the cave sediments of Indo-Burma biodiversity hotspot[J].Environmental Science and Pollution Research,2016,3(12):12216-12226
    [19] 吕志堂,纪翠平,苏强,等.3株反硝化聚磷菌的分离与鉴定[J].环境工程学报,2009,3(8):1405-1408
    [20] WANG Q, MA F, WEI L, et al.Screen and characteristics of a denitrifying phosphorus-removal bacteria[J].Journal of Biotechnology,2008,6(4):690-691
    [21] CAI T M, CHEN L W, WU S Z, et al.Selection of denitrifying phosphorus-removing bacteria and its characteristic[J].Environmental Science,2010,1(10):2487-2492
    [22] MA F, YANG F F, LI A, et al.Biological characteristics of denitrifying polyphosphate-accumulating organisms[J].Environmental Science,2011,2(9):2710-2715
    [23] LIU J, LU Z, ZHANG J, et al.Phylogenetic characterization of microbial communities in a full-scale vermifilter treating rural domestic sewage[J].Ecological Engineering,2013,1:100-109
    [24] 杨启瑞,范红兵.微生物生长曲线测定方法的改进[J].微生物学研究与应用, 1991(4):23-25
    [25] 吉果,陈启伟,宫晓炜,等.常用抗生素对鸭疫里默氏杆菌MIC最佳试验条件的确立[J].黑龙江畜牧兽医, 2017(5):32-36
    [26] 崔玉波,朱宝英.低温下ABR酸化反应器的运行效果及微生物活性研究[J].环境工程学报, 2007,1(2):49-53
    [27] 李辉,方正.污泥接种比例对微生物燃料电池的影响[J].武汉大学学报(工学版), 2014,7(2):234-238
    [28] 陈磊.反硝化聚磷菌培养驯化分离方法及菌种特性的研究[D].济南:山东大学, 2008
    [29] 洪武林.反硝化聚磷菌的分离鉴定及其固定化应用研究[D].上海:东华大学, 2012
    [30] 罗斯,赵玉莲.环境对微生物活性的影响:上[J].上海调味品,1987(4):21-24
    [31] 武春燕, 卜玉山.不同有机物添加接种物产甲烷差异分析[J].环境工程学报, 2017,1(2):1147-1152
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  2643
  • HTML全文浏览数:  2258
  • PDF下载数:  377
  • 施引文献:  0
出版历程
  • 刊出日期:  2018-02-08
吴晓娜, 王助贫, 谢恩, 任梦丽, 刘婷婷, 郑蕾, 丁爱中. 一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响[J]. 环境工程学报, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224
引用本文: 吴晓娜, 王助贫, 谢恩, 任梦丽, 刘婷婷, 郑蕾, 丁爱中. 一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响[J]. 环境工程学报, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224
shu
WU Xiaona, WANG Zhupin, XIE En, REN Mengli, LIU Tingting, ZHENG Lei, DING Aizhong. Screening of one strain of denitrifying phosphorus accumulation bacteria (DPAB) and inhibitions effects of nitrogen-phosphorus removal[J]. Chinese Journal of Environmental Engineering, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224
Citation: WU Xiaona, WANG Zhupin, XIE En, REN Mengli, LIU Tingting, ZHENG Lei, DING Aizhong. Screening of one strain of denitrifying phosphorus accumulation bacteria (DPAB) and inhibitions effects of nitrogen-phosphorus removal[J]. Chinese Journal of Environmental Engineering, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224
shu

一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响

  • 1. 北京师范大学水科学研究院,北京100875
  • 2. 北控水务集团有限公司,北京 100102
  • 3. 中国农业大学水利与土木工程学院,北京 100083
基金项目:

北京市科技计划项目(D161100000216001)

国家自然科学基金资助项目(51509003)

摘要: 以环形推流一体化氧化沟反应器厌氧段污泥为接种污泥,通过吸磷实验、硝酸盐还原产气实验、异染颗粒染色实验及PHB染色实验从接种污泥中筛选得到一株能实现同步脱氮除磷菌株N-12；经过16S rDNA测序鉴定,确定该菌株为不动杆菌属(Acinetobacter)。绘制该菌株的生长曲线,并设置0%、1%、2%、5%、10%和15%等6种不同初始接种比,以接种比作为微生物生物量的衡量参数,研究不同接种比对反硝化聚磷菌脱氮除磷效应的影响,确定菌株最佳接种比。实验结果表明:初始接种比2%体系的脱氮除磷效果最好；利用SPSS statistics 19统计软件对磷酸盐去除率、硝态氮去除率、OD600值及pH进行相关性分析,表明磷酸盐去除率和硝酸盐去除率显著正相关(Person相关系数=0.826,P600值及pH值都显著正相关。

English Abstract

参考文献 (31)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心