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2种温度下污泥水富集硝化菌的对比

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于莉芳, 傅学焘, 杨秀玲, 滑思思, 冯云堂. 2种温度下污泥水富集硝化菌的对比[J]. 环境工程学报, 2018, 12(1): 102-109. doi: 10.12030/j.cjee.201705103
引用本文: 于莉芳, 傅学焘, 杨秀玲, 滑思思, 冯云堂. 2种温度下污泥水富集硝化菌的对比[J]. 环境工程学报, 2018, 12(1): 102-109. doi: 10.12030/j.cjee.201705103
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YU Lifang, FU Xuetao, YANG Xiuling, HUA Sisi, FENG Yuntang. Comparison of nitrifier accumulation in sludge liquor treatment system at two different temperatures[J]. Chinese Journal of Environmental Engineering, 2018, 12(1): 102-109. doi: 10.12030/j.cjee.201705103
Citation: YU Lifang, FU Xuetao, YANG Xiuling, HUA Sisi, FENG Yuntang. Comparison of nitrifier accumulation in sludge liquor treatment system at two different temperatures[J]. Chinese Journal of Environmental Engineering, 2018, 12(1): 102-109. doi: 10.12030/j.cjee.201705103
shu

2种温度下污泥水富集硝化菌的对比

  • 1.

    西安建筑科技大学环境与市政工程学院,西安710055

  • 2.

    中山市春兴建筑科技有限公司,中山 528400

  • 基金项目:

    国家自然科学基金青年基金资助项目(51208414)

    陕西省教育厅专项(12JK0650)

Comparison of nitrifier accumulation in sludge liquor treatment system at two different temperatures

  • 1.

    School of Environmental & Municipal Engineering, Xi′an University of Architecture and Technology, Xi′an 710055, China

  • 2.

    Zhongshan Chunxing Construction Technology Co.Ltd., Zhongshan 528400, China

  • Fund Project:

  • 摘要: 污泥水富集硝化菌添加强化硝化工艺可以在处理高温、高浓度氨氮污泥水的同时富集硝化菌并添加至城市污水处理系统强化硝化,但硝化菌对温度敏感,温度将对硝化菌的富集效果产生影响。研究在高氨氮负荷(1.20 kg·(m3·d)-1)下运行SBR以富集硝化菌,对比2种温度(25 ℃、30 ℃)下富集硝化菌的群落结构和动力学特性。荧光原位杂交结果表明,硝化菌r-决策者(Nitrosomonas europaea,Nitrobacter)的份额随温度升高而逐渐增加。硝化动力学参数测定发现,随温度升高,AOB活性增加,基质半饱和常数增加；而NOB则相反,这主要是因为温度升高,反应器中最大游离氨(FA)、游离亚硝酸(FNA)浓度增加,而NOB比AOB更容易受FA、FNA的抑制。此外,温度对AOB与NOB的溶解氧半饱和常数无明显影响。
    Abstract: Sludge liquor (reject water) is one of the by-products of the water resource recovery facilities (WRRFs), it has relative high temperature, high ammonia concentration and may increase the nitrogen loading of the WRRFs. Bioaugmentation with nitrifiers enriched by sludge liquor is a promised way to enhance nitrification performance of the water resource recovery facilities (WRRF). While nitrifiers are temperature sensitive, the effect of nitrifier enrichment may affected by temperature. In this study, nitrifiers were accumulated in an SBR at high ammonia loading (1.20 kg· (m3·d)-1) at 25 ℃ and 30 ℃, then the enriched nitrifier community and the kinetic performances were investigated by fluorescence in situ hybridization (FISH) and respirometric assays. FISH results showed that r-strategists (Nitrosomonas europaea,Nitrobacter) increased with temperature. The kinetic parameters showed that the maximum substrate utilization rate and the half saturation constant for substrate of ammonia oxidized bacteria (AOB) increased with temperature, while the opposite trend was shown with nitrite oxidized bacteria (NOB) mainly due to severe free nitrous acid (FNA) inhibition under high temperature. Besides, it didn’t have significant difference between the half saturation constant for oxygen of nitrifiers at 25 ℃ and 30 ℃.
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  • 刊出日期:  2018-01-14

2种温度下污泥水富集硝化菌的对比

  • 1. 西安建筑科技大学环境与市政工程学院,西安710055
  • 2. 中山市春兴建筑科技有限公司,中山 528400
基金项目:

国家自然科学基金青年基金资助项目(51208414)

陕西省教育厅专项(12JK0650)

摘要: 污泥水富集硝化菌添加强化硝化工艺可以在处理高温、高浓度氨氮污泥水的同时富集硝化菌并添加至城市污水处理系统强化硝化,但硝化菌对温度敏感,温度将对硝化菌的富集效果产生影响。研究在高氨氮负荷(1.20 kg·(m3·d)-1)下运行SBR以富集硝化菌,对比2种温度(25 ℃、30 ℃)下富集硝化菌的群落结构和动力学特性。荧光原位杂交结果表明,硝化菌r-决策者(Nitrosomonas europaea,Nitrobacter)的份额随温度升高而逐渐增加。硝化动力学参数测定发现,随温度升高,AOB活性增加,基质半饱和常数增加；而NOB则相反,这主要是因为温度升高,反应器中最大游离氨(FA)、游离亚硝酸(FNA)浓度增加,而NOB比AOB更容易受FA、FNA的抑制。此外,温度对AOB与NOB的溶解氧半饱和常数无明显影响。

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