不同植物-微生物联合修复体系下石油烃的降解
Degradation of petroleum hydrocarbons under different plant-microbial remediation system
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摘要: 石油烃作为环境中广泛存在的有机污染物之一,对人体健康造成严重的危害。以位于天津的大港油田原油污染土壤中筛选出的耐低温高效石油烃降解菌为供试菌株,以小麦、紫花苜蓿作为供试植物,比较不同类型植物以及不同的外源菌接种方式对石油降解的影响,并采用荧光素比色法分析荧光素二乙酸酯(FDA)酶活性随时间的变化规律。经过70 d的降解,原状土壤的总石油烃含量从30 720 mg·kg-1下降为26 800 mg·kg-1,降解率为12.76%。相比于小麦,紫花苜蓿对石油烃的降解具有更好的促进效果,降解率为24.85%。接种菌悬液后再种植植物时,石油烃降解效果接近于单独接种菌悬液处理。小麦-固定化外源菌处理条件下,降解率为21.10%,实验后期石油烃的降解速率远远高于其他处理,表现出良好的修复潜力。FDA酶活性经历了先下降、后上升再下降直至平缓的过程,并且受到种植植物和投加外源菌的影响。Abstract: As one of the organic pollutants widely present in the environment, petroleum hydrocarbon is harmful to human health. Low temperature-resistance and high efficient petroleum hydrocarbon degrading bacteria screened from crude oil contaminated soil in Dagang Oil Field was selected as target cells. Wheat and alfalfa were used as tested plants. The effects of different plant types and different inoculation methods on oil degradation were compared.We used fluorescein assay to analyze fluorescein acetate (FDA) enzyme activity changes with time. After 70 days of degradation, the total petroleum hydrocarbon content of undisturbed soil sample decreased from 30 720 mg·kg-1 to 26 800 mg·kg-1, the degradation rate was up to 12.76%. Compared with wheat, alfalfa had a better effect on the degradation of petroleum hydrocarbons, the degradation rate was 24.39%. Under the condition of wheat immobilized bacteria, the degradation rate was 21.10%, and the degradation rate of petroleum hydrocarbon was much higher than that of other treatments in the later stage, which showed good potential for recovery. The activity of FDA was decreased first, then increased and then decreased until it was stable, and affected by both plant and exogenous bacteria.
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Key words:
- petroleum hydrocarbon /
- wheat /
- alfalfa /
- bacterial suspension /
- immobilized bacteria /
- FDA enzyme activity
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[1] 马强, 林爱军, 马薇,等.土壤中总石油烃污染(TPH)的微生物降解与修复研究进展[J].生态毒理学报,2008,3(1):1-8 [2] 倪妮, 宋洋, 王芳,等.多环芳烃污染土壤生物联合强化修复研究进展[J].土壤学报,2016,53(3):561-571 [3] 刘五星, 骆永明, 滕应,等.我国部分油田土壤及油泥的石油污染初步研究[J].土壤,2007,39(2):247-251 [4] 刘志培, 刘双江.我国污染土壤生物修复技术的发展及现状[J].生物工程学报,2015,31(6):901-916 [5] GUTIERREZ-GINES M J,HERNANDEZ A J,PEERZ-LEBLIC M I,et al.Phytoremediation of soils co-contaminated by organic compounds and heavy metals: Bioassays with Lupinus luteus L.and associated endophytic bacteria[J].Journal of Environmental Management,2014,143(10):197-207 [6] 邓振山, 王阿芝, 孙志宏,等.利用植物-根际菌协同作用修复石油污染土壤[J].西北大学学报(自然科学版),2014,44(2):241-247 [7] XU Y, SUN G D, JIN J H, et al.Successful bioremediation of an aged and heavily contaminated soil using a microbial/plant combination strategy[J].Journal of Hazardous Materials,2014,264(2):430-438 [8] SWISHER R, CARROLL G C.Fluorescein diacetate hydrolysis as an estimator of microbial biomass on coniferous needle surfaces[J].Microbial Ecology,1980,6(3):217-226 [9] SCHNURER J,ROSSWALL T.Fluorescein diacetate hydrolysis as a measure of total microbial activity in soil and litter[J].Applied & Environmental Microbiology,1982,43:1256-1261 [10] ADAM G,DUNCAN H.Development of a sensitive and rapid method for the measurement of total microbial activity using fluorescein diacetate (FDA) in a range of soils[J].Soil Biology & Biochemistry,2001,33(7/8):943-951 [11] GREEN V S,STOTT D S, DIACK M.Assay for fluorescein diacetate hydrolytic activity: Optimization for soil samples[J].Soil Biology & Biochemistry,2006,38(4):693-701 [12] 马星竹.长期施肥土壤的FDA水解酶活性[J].浙江大学学报(农业与生命科学版),2010,36(4):451-455 [13] 王校常, 陆琴, 李腊梅,等.太湖地区典型水稻土FDA水解酶活性的剖面分布特征[J].植物营养与肥料学报,2006,12(6):834-839 [14] 李振高, 骆永明, 滕应.土壤与环境微生物研究法[M].北京:科学出版社,2008 [15] 蒋炳伸, 李鸿雁, 杨喜田.应用荧光素二乙酸脂水解酶活性研究不同绿地土壤微生物活性[J].河南农业科学,2011,40(8):130-133 [16] HE W, HUANG Y, ZHU M, et al.Effect of Hg and Cd on soil urease activity[J].Acta Pedologica Sinica,2002,39(3):412-420 [17] 王红旗, 石油烃污染土壤的微生物修复技术及应用[M].北京:中国环境科学出版社,2015 [18] 程国玲, 李培军.矿物油对小麦、苜蓿种子萌发和生长的影响[J].种子,2007,26(6):24-27 [19] 宋玉芳, 许华夏, 任丽萍,等.土壤重金属对白菜种子发芽与根伸长抑制的生态毒性效应[J].环境科学,2002,23(1):103-107 -
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